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. 2021 Sep 2;17(9):e1009888. doi: 10.1371/journal.ppat.1009888

Fig 6. Galectins are recruited to R. equi.

Fig 6

(A) Immunofluorescence (IF) of RAW 264.7 cells stably expressing 3XFLAG (FL)-tagged galectin-3, -8, or -9 infected with GFP expressing R. equi 103+ (MOI-5) for the indicated times. (B) Quantification of galectin-1, -3, -8, or -9 recruitment to R. equi at the indicated times. (C) Quantification of galectin-1, -3, -8, or -9 puncta in R. equi-infected RAW 264.7 cells at the indicated times. A minimum of 100 cells were quantified per coverslip. (D) IF of RAW 264.7 cells stably expressing 3X FL-tagged galectin-8 infected with GFP expressing R. equi 103+ or 103- (MOI-5) for the indicated time and quantification of galectin-8 positive R. equi in at 8h expressed as percent of total R. equi. IF images are representative of 3 independent experiments. Quantification is the percent of positive bacteria with at least 100 bacteria quantified per coverslip. Error bars are ±SEM. Statistical significance was determined using Students’ t-test. *p < 0.05, n.s. = not significant.