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(a) Schematic of our multifocus imaging system. (b)–(d) Construction of 3-, 6-, and 9-plane z-splitter prisms. (e)–(g) Fitted axial intensity profile of a 1 μm fluorescent bead measured with 3-, 6-, and 9-plane prisms. (h)–(j) Theoretical and measured axial focal positions associated with 3-, 6-, and 9-plane prisms. (k) Simultaneous 9-depth transmission imaging of a USAF resolution target using a 9-plane prism. Δz = 22.1 μm.
