|
Reagent type (species) or resource
|
Designation
|
Source or reference
|
Identifiers
|
Additional information
|
| Strain, strain background (Mus musculus, both sexes) |
Kmt2d+/βGeo mice (fully backcrossed to C57BL/6J) |
Originally from Bay Genomics and described in PMID:2527309625273096. |
Kmt2d+/βGeo,Mll2Gt(RRt024)BygRRID:MGI:5829565
|
A previously characterized mouse model of Kabuki syndrome (type 1). |
| Strain, strain background (Mus musculus, females only) |
Kdm6a± mice (fully backcrossed to C57BL/6J, observed male lethality) |
Ordered from EMMA (European Mouse Mutant Archive) |
Kdm6a+/, Kdm6atm1d(EUCOMM)Wtsi
MGI:4434460
|
A previously characterized mouse model of Kabuki syndrome (type 2). Transition from Kdm6atm1a(EUCOMM)Wtsi to Kdm6atm1d(EUCOMM)Wtsi performed in Bjornsson laboratory. |
| Strain, strain background (Mus musculus, both sexes) |
Crebbp±mice (fully backcrossed to C57BL/6J) |
Ordered from Jackson laboratory and described in PMID:10673499
|
Crebbp+/-Crebbptm1Dli, RRID:MGI:2175793
|
A previously characterized mouse model of Rubinstein-Taybi syndrome (type 1). |
| Sequence-based reagent |
βGeo F |
This paper |
PCR primers |
CAAATGGCGATTACCGTTGA
|
| Sequence-based reagent |
βGeo R |
This paper |
PCR primers |
TGCCCAGTCATAGCCGAATA
|
| Sequence-based reagent |
Tcrd (control) F |
This paper |
PCR primers |
CAAATGTTGCTTGTCTGGTG
|
| Sequence-based reagent |
Tcrd (control) R |
This paper |
PCR primers |
GTCAGTCGAGTGCACAGTTT
|
| Sequence-based reagent |
Kdm6aTm1c F |
This paper |
PCR primers |
AAGGCGCATAACGATACCAC
|
| Sequence-based reagent |
Kdm6aTm1c, Floxed LR |
This paper |
PCR primers |
ACTGATGGCGAGCTCAGACC
|
| Sequence-based reagent |
Tcrd (control) F- |
This paper |
PCR primers |
CAAATGTTGCTTGTCTGGTG
|
| Sequence-based reagent |
Tcrd (control) R |
This paper |
PCR primers |
GTCAGTCGAGTGCACAGTTT
|
| Sequence-based reagent |
CrebbpR-T F |
This paper |
PCR primers |
TAAGCAGCAGCATCCTTTGG
|
| Sequence-based reagent |
CrebbpR-T_WT |
This paper |
PCR primers |
CCTGACAATGTGTCATGTGAT
|
| Sequence-based reagent |
CrebbpR_T_MUT R: |
This paper |
PCR primers |
ATGCTCCAGACTGCCTTGGGA
|
| Commercial assay or kit |
IgA ELISA kit |
Thermo |
Catalog # EMIGA |
|
| Commercial assay or kit |
CD19 positive selection |
Miltenyi |
130-052-201 |
|
| Commercial assay or kit |
Tagmentation |
IlluminaNextera |
FC-121–1030 |
|
| Commercial assay or kit |
Digitonin |
Promega |
G9441 |
|
| Commercial assay or kit |
DNA clean and concentration kit |
Zymo |
D4013 |
|
| Commercial assay or kit |
Select A size purification |
Zymo |
D4080 |
|
| Commercial assay or kit |
DNA high sensitivity |
Agilent |
5067–4626 |
|
| Commercial assay or kit |
Qubit dsDNA HS |
Thermo |
Q32851 |
|
| Commercial assay or kit |
Direct-zol RNA microprep |
Zymo |
R2060 |
|
| Commercial assay or kit |
Quant-iT RiboGreen |
Thermo |
R11490 |
|
| Commercial assay or kit |
RNA HS Assay kit |
Thermo |
Q32852 |
|
| Commercial assay or kit |
RNA 6000 Pico |
Agilent |
5067–1513 |
|
| Commercial assay or kit |
NEBNext Poly(A) mRNA isolation module |
New England Biolabs |
E7490 |
|
| Commercial assay or kit |
NEBNext Ultra II Directional RNA Library Prep kit |
New England Biolabs |
E7760/E7765 |
|
| Commercial assay or kit |
KAPA library Quantification kit |
KAPA |
KK4824 |
|
| Software, algorithm |
BowTie2 |
PMID:22388286
|
RRID:SCR_016368
|
Default parameters |
| Software, algorithm |
Samtools |
PMID:19505943
|
RRID:SCR_002105
|
|
| Software, algorithm |
MACS2 |
PMID:18798982
|
RRID:SCR_013291
|
Keep-dup = all |
| Software, algorithm |
DESeq2 |
PMID:25516281
|
RRID:SCR_015687
|
|
| Software, algorithm |
Surrogate Variable Analysis |
PMID:17907809
|
RRID:SCR_012836
|
|
| Software, algorithm |
Salmon |
PMID:28263959
|
V0.10RRID:SCR_017036
|
|
| Other |
GEO submission of all data |
Accession GSE162181
|
RRID:SCR_005012
|
|
