Figure 5.

Differential signaling engagement of CD105^pos and CD105^neg PaFs

(A) MC analysis of CD105^pos and CD105^neg PaFs signaling. Data are displayed as median mass intensities (MMI) and column Z scores. Specific phosphorylation sites are annotated in brackets.

(B–D) RNA-seq analysis of CD105^pos and CD105^neg PaFs stimulated as displayed for 6 h (n = 3). DEGs were identified using DEseq2 with Benjamini-Hochberg adjusted p < 0.05. Data are displayed as Venn diagrams (top), with example genes listed (below). Unique DEGs of CD105^pos PaFs in red, CD105^neg PaFs in blue, and shared in purple. Numbers of significant DEGs are displayed in parenthesis.

(E and F) Expression of myCAF (E) and iCAF (F) genes from CD105^pos and CD105^neg PaFs stimulated with TGF-β1 or IL-1α (n = 4) for 3 days. Eng expression is also shown. Data displayed as mean ± SD.

Samples were compared using unpaired t tests (E and F). ns, not significant; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

See also Figure S6 and Table S5.