Figure 1.

Wild-type cells are eliminated by apoptosis from co-cultures with variant hPSCs

(A–B) Growth curves of wild-type-RFP and variant-GFP cells grown separately (A), or in co-culture (B). Fields acquired: entire well.

(C) Representative images of wild-type-RFP hPSCs (red) grown separately (upper panels) or in co-culture with variant-GFP hPSCs (green) (lower panels). Scale bar: 50 μm.

(D) Ratio of variant-GFP/wild-type-RFP cells in separate versus co-culture conditions.

(E) Percentage of cells positive for cleaved caspase-3.

Data are the mean of three independent experiments ± SD. n.s. nonsignificant; ^∗∗∗∗p < 0.0001, two-way ANOVA, followed by Holm-Sidak’s multiple comparisons test.

See also Figures S1, S2, and S3; Video S1.