Figure 4.

The winner phenotype is dependent on higher proliferative rates of variant cells

(A–E) Growth curves of wild-type-RFP and v1q cells (A), wild-type-RFP and v20q cells (B), V1,17q,i20 and variant-GFP cells (C), v1q and variant-GFP cells (D), and v20q and variant-GFP cells (E) grown separately or in co-culture. Cells in (A, B, D, and E) were plated at 45,000 cells/cm². Cells in (C) were plated at the lower density of 22,500 cells/cm² due to the faster growth rate of both sublines with a complex karyotype. Fields acquired: entire well. Data represent the mean of two independent experiments (A, B, D, and E) or the mean of 6 technical replicates from the same experiment (C) ± SD.

(F–G) Percentage of cells positive for cleaved caspase-3 in v1q and variant-GFP cells (F) and v20q and variant-GFP cells (G) in separate cultures or upon co-culture. Data are the mean of three independent experiments ± SD.

(H) Western blot of antiapoptotic proteins in wild-type, v1q, v20q, and variant-GFP cells. β-actin was used as a loading control.

Statistical analysis was performed by two-way ANOVA, followed by Holm-Sidak’s multiple comparisons test (A, B, D, E, F, and G) or Student’s t test (C); n.s. nonsignificant; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.