Figure 7.
Optimized culture conditions restrict the competitive behavior of winner hPSCs
(A and B) Nuclear to cytoplasmic ratio of YAP (A), and the mean intensity of p-YAP in the cytoplasm (B) of wild-type and variant-GFP hPSCs in co-culture ± 1μM (R)-PFI-2.
(C–E) Ratio of H7 variant-GFP/wild-type (C), H14 variant-BJ1/H14 wild-type (D), and HUES-17 V12,17/HUES-17 wild-type cells (E) in separate culture, co-culture and co-culture treated with 1μM (R)-PFI-2 at increasing plating cell density.
(F–H) Number of wild-type H7 (F), H14 (G), and HUES-17 (H) cells on day 3 of co-culture in control or 1μM (R)-PFI-2 conditions at increasing plating cell density. Fields acquired:16. Data are the mean of two (C and F) or three (D, E, G, and H) independent experiments ± SD. n.s. nonsignificant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, two-way ANOVA followed by Fisher’s LSD test.