Figure 4.

Effect of EO crude fruit extract on NO production and iNOS mRNA expression in BV2 and differentiated Neuro2a cells. Both BV2 and differentiated Neuro2a cells were treated with LPS (1 μg/mL) or EO crude fruit extract (0.5 or 1 μg/mL). After 24 h, the supernatant was collected, and NO levels were assessed using the Griess assay. Cells were collected to evaluate the iNOS mRNA expression levels. (a) BV2 cells, (b) differentiated Neuro2a cells, and (c) iNOS mRNA expression in BV2 cells were tested by RT-qPCR ^∗p < 0.05 (ANOVA; significant at ^∗∗p < 0.01 and ^∗∗∗p < 0.001, compared with the LPS-treated group.)