Figure 5.

Genetic GPVI deficiency potentiates lymphatic blood filling in Gp1ba-Cre CLEC-2-deficient mice.Gross images were scored as described in the methods to generate an assessment of blood filling of lymphatic vessels (Ai) and bleeding in lymph nodes (Bi) in GPVI knockout and Gp1ba-Cre CLEC-2/GPVI double-deficient mice (aged between 8 and 20 weeks). Gp1ba-Cre CLEC-2/GPVI double-deficient mice had a significantly higher score than Gp1ba-Cre CLEC-2-deficient mice for both lymphatic vessels and lymph nodes. GPVI-deficient mice showed no evidence of lymphatic vessel blood filling but did show evidence of bleeding in lymph nodes. Gross images were blinded before being scored by two independent researchers. Bars represent median, error bars represent the interquartile range and dots represent individual animals. The black dotted line indicates the median score of Gp1ba-Cre CLEC-2-deficient mice. Stars indicate the statistical difference between Gp1ba-Cre CLEC-2 and CLEC-2/GPVI deficient mice, which was assessed by Mann–Whitney U test. (Aii) Images representative of n = 12. Abbreviations: SE = sub-epigastric collector (indicated by yellow arrow); scale bar = 250 μm, TD = thoracic duct (indicated by yellow arrow); scale bar = 500 μm, Arc = intestinal arcade; scale bar = 1 mm. (Bii) Images representative of n = 12. Abbreviations: ILN = inguinal lymph node; scale bar = 1 mm, MLN = mesenteric lymph nodes; scale bar = 1 mm.