Figure 6.

The lymphatic blood filling phenotype of platelet conditional CLEC-2-deficient mice does not progress after development.Gross images of conditional CLEC-2-deficient mice of two age groups (1 month or younger but weaned, and 3 months or older) and were scored as described in the methods to generate an assessment of blood filling of lymphatic vessels (A) and bleeding in lymph nodes (B). There was no statistical difference between the age groups within a strain for the lymphatic vessel score; however, the lymph node score increased in the older age groups of Gp1ba-Cre CLEC-2-deficient and Gp1ba-Cre CLEC-2/GPVI double-deficient mice (n = 8 for all strains). (C) GPVI-deficient mice also showed a significant increase in lymph node score in mice aged 4 months or over when compared to those 1 month or younger, but weaned (n = 6). Bars represent median, error bars represent the interquartile range and dots represent individual animals. The black dotted line indicates the maximum score possible. Significance assessed by Mann–Whitney U test for each strain.