Figure 1. Experimental design, analytic workflow, and auditory-responsive ROIs.

(A) Schematic of the imaging and experimental setup used to observe brain activity from zebrafish larvae, showing the mini-speaker attached to the back coverslip of the chamber. (B) Summary of the analysis workflow used to identify and categorize auditory-responsive neurons across the brain. (C, top) shows a schematic of the stimulus train used, including stimuli with a range of different forms, frequencies, and amplitudes. (C, bottom) shows the mean response of all the auditory-responsive ROIs in the resulting dataset. (D and E) All auditory-responsive ROIs across the brain are shown with the strength of the response represented by the color, and the correlation to the linear regression model (r² value) represented as the size of the sphere. The strength of the response is between 2 (the threshold for inclusion) and 12SD. Brain regions containing a high proportion of auditory-responsive ROIs are identified spatially (Inset, D). Tel: telencephalon, Th: thalamus, Tec: tectum, TS: torus semicircularis, Teg: tegmentum, Cb: cerebellum, ON: octavolateralis nucleus, HB: remaining hindbrain. R, rostral; C, caudal; D, dorsal, V, ventral here and in subsequent figures. Scale bar applies to both D and E.