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. 2021 Sep 15;11:18309. doi: 10.1038/s41598-021-97844-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PBP2a_mecA protein detection using in-source generated peptide-like fragments and MS/MS fragmentation. (a, b) Source-induced dissociated N-terminal peptide-like fragments from PBP2a_mecA (ATCC MRSA isolate BAA-44) at precursor m/z 596.8961 (a) and m/z 653.4382 (b) for selected ion monitoring (insets) and associated representative targeted MS/MS spectra (n ≥ 100). (c) Synthetic peptide confirmation of source-induced dissociated peptide-like precursor m/z 596.8961 and associated MS/MS spectrum (inset spectrum series displays peptide spectrum without in-source energy, with in-source dissociation, and magnified spectrum of target precursor following in-source dissociation). (d) Synthetic peptide confirmation of source-induced dissociated peptide-like precursor m/z 653.4382 and associated MS/MS spectrum (inset spectrum series displays peptide spectrum without in-source energy, with in-source dissociation, and magnified spectrum of target precursor following in-source dissociation). Data collection for synthetic peptides was repeated multiple times on different MS instruments (n = 3).