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. 2021 Sep 15;11:18309. doi: 10.1038/s41598-021-97844-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of source-induced peptide detection of PBP2a across representative MRSA panel and clinical isolates. (a) Performance of source-induced detection method for PBP2a_mecA (top panel) and PBP2a_mecC (bottom panel) N-terminal peptide-like fragments for MRSA strains exhibiting different SCCmec and PFGE genetic backgrounds, as well as negative MSSA isolates (listed in Supplemental Table S6). Strains were cultured on TSA plates and harvest after overnight growth. Colored bars represent the mean maximum intensity for each product ion over a 60-min LC protein separation. Results are from distinct biological replicates (n = 3). (b) Performance of MSSA and MRSA quality control strains along with clinical isolates over five-minute PBP2a_mecA detection method using custom SPE trap and source-induced dissociation method. Strains were cultured on Blood Agar plates and harvested after overnight growth. Data are the mean maximum ion intensity for each product ion and calculated from distinct biological replicates (n = 2 or 3).