Skip to main content
. 2021 Sep 15;12:5463. doi: 10.1038/s41467-021-25550-2

Fig. 4. The loss of the aggregation of the WT Δ133p53β upon interaction with p63 family members.

Fig. 4

a Co-immunoprecipitation analysis of WT Δ133p53α and WT Δ133p53β interaction with endogenous p53 upon expression in MCF-7 cells. n = 5. b Co-immunoprecipitation analysis of mutated Δ133p53α and Δ133p53β interaction with endogenous p53 upon expression in MDA-MB-231 D3H2LN cells. n = 5. c Co-immunoprecipitation analysis of mutated Δ133p53α and Δ133p53β interaction with endogenous p53 upon expression in SK-BR-3 cells. n = 5. d Co-immunoprecipitation analysis of WT Δ133p53β and ΔNp63α interaction upon co-expression in H1299 cells. n = 3. e Western blot analysis of the aggregate forming capacity of WT Δ133p53β in the soluble protein fraction from H1299 cells upon co-expression with ΔNp63α protein. n = 3. f Western blot analysis of the aggregate forming capacity of WT Δ133p53β in the insoluble protein fraction from H1299 cells upon co-expression with ΔNp63α protein. n = 3. g Immunofluorescent analysis of WT Δ133p53β aggregates after co-expression with ΔNp63α in H1299 cells upon 3D reconstruction of the confocal Z-stacks. Red: α-Flag (Δ133p53β), green: ΔNp63α, blue: Hoechst. Scale bar 8 μm. n = 3. h Co-immunoprecipitation analysis of WT Δ133p53β and TAp63α interaction upon co-expression in H1299 cells. n = 3. i Western blot analysis of the aggregate forming capacity of WT Δ133p53β in the soluble protein fraction from H1299 cells upon co-expression with TAp63α protein. n = 3. j Western blot analysis of the aggregate forming capacity of WT Δ133p53β in the insoluble protein fraction from H1299 cell line upon co-expression with TAp63α protein. n = 3. The arrows indicate aggregates. Molecular weight is expressed in kDa. Source data are provided as a Source Data file.