Figure 5.

Effects of L-NAME administration. (A) Echocardiographic data analyzing fractional shortening (FS) or left ventricular systolic dimension (LVDs) of mice subjected to TAC + EMPA with or without L-NAME administration (n = 10, 4). (B) TUNEL (terminal deoxynucleotidal transferase–mediated biotin–deoxyuridine triphosphate nick-end labeling) of cardiac tissues of mice subjected to TAC + EMPA with or without L-NAME administration (arrow indicates TUNEL-positive ECs, scale bar = 10 μm) (right panel indicates quantification of TUNEL positive ECs (n = 6, 10)) (C) Immunofluorescence study for IB4-isolectin in cardiac tissues of indicated mice. Right panels indicates quantified data demonstrated as vessel density (estimated as the number of microvessels relative to the number of cardiomyocytes/cross sectional area (CSA) (μm²)) (n = 5, 10. scale bar = 100 μm). Data were analyzed by a 2-tailed Student’s t test (A–C). *P < 0.05, **P < 0.01. Values are shown as the mean ± SEM.