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. 2021 Sep 15;11:18368. doi: 10.1038/s41598-021-96379-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative infrared and visible maps of a fibrillary plaque (left panels) and an Oligomeric/Granullar plaque (right panels). (Top row) Images in the visible region of the plaque labeled with an antiamyloid antibody in a contiguous brain slide; (Midle row) absorbance ratios corresponding to fibrillary β-sheet amyloid aggregation; (Bottom row) absorbance ratios corresponding to antiparallel β-sheet aggregation distribution. Ratios were calculated from the second derivative spectra. Scale bar = 10 μm. Opus 7.5 (Bruker) software was used for the maps representation.