Figure 1.

Differences in the protein expression of cannabinoid receptor 1 (CB₁), CB₂ and phospho and total mammalian target of rapamycin (mTOR) at Ser2448, phospho and total forkhead box O (FOXO)3a at Thr32, microtubule-associated protein 1A/1B-light chain 3 (LC3b), muscle RING finger-1 (MuRF1), MyoD and Pax7 between skeletal muscle of young (n = 18) and old adults (n = 14). Protein targets were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH), vinculin or the total protein form. Data are shown as mean (bar graph in grey) with individual data points (black dots superimposed on the bar graphs). *p < 0.05 and ^†p = 0.05–0.10 for Independent-Samples t Test or Mann–Whitney U Test. Raw western blot data of all replicates are displayed in Supplementary Figure 2. Ponceau red staining of the presented western blots are included in Supplementary Figure 3.