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. 2021 May 10;15(10):3076–3083. doi: 10.1038/s41396-021-00927-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Differentiation based on pairwise average nucleotide identity. B Differentiation based on pairwise average F_ST in 2496 orthologous genes. Colour bars represent host genotypes (red: B. puteoserpentis, yellow: hybrids) and the sampling year (light grey: 1997, dark grey: 2001). Turquoise indicates a higher differentiation or more dissimilar genomes. Based on a Mantel test, neither clustering based on ANI (A), nor F_ST (B) correlated with host pairwise genetic distances based on 18 SNP markers (A r = 0.054, p = 0.222, B r = 0.006, p = 0.435) or sampling year (A r = −0.191, p = 0.949, B r = 0.105, p = 0.150) (colour figure online).