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. 2021 Apr 26;15(10):2892–2905. doi: 10.1038/s41396-021-00984-0

Fig. 4. Sis-EVs support heterotrophic growth of Sulfolobus cells.

Fig. 4

a Growth curves of S. islandicus REY15A in MV (M: mineral salts, V: vitamin mix) solution lacking organic carbon and nitrogen sources; MV solution supplemented with 1.5 ml preparations containing different amounts of EVs (MV + EVs): 24.8 μg for I, 49.6 μg for II, and 74.4 μg for III; and rich medium (MTSV), which in addition to MV solution contains 0.2% (wt/vol) sucrose (S) and 0.2% (wt/vol) tryptone (T). There was significant increase (two paired t test, p < 0.05) in optical density (OD600) of REY15A cells in the MV solution supplemented with EVs (III), as compared to the control culture lacking EVs. b Growth curves of S. islandicus REY15A in MSV (M: mineral salts, S: sucrose, V: vitamin mix) medium lacking organic nitrogen source; MSV medium supplemented with different concentrations of EVs (MSV + EVs), and rich MTSV medium. There was significant increase (two paired t test, p < 0.05) in optical density (OD600) of REY15A cells in the MSV medium supplemented with EVs, as compared to the control culture lacking EVs. Error bars represent standard deviations from three independent experiments.