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. 2021 Sep 8;10(9):bio058461. doi: 10.1242/bio.058461

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — GFP expression efficiency in human MSCs after transduction with AAV-DJ or AAV-2. (A) top panels show representative fluorescence photomicrographs of transduced cells (green channel) at 100× magnification; lower panels shows the same field of view in phase contrast. (B) Typical flow cytometry histograms showing GFP expression in human MSCs transduced with AAV2 or AAV-DJ from 4th to 21st day. The percentage of GFP-positive cells in intact MSC (control) or transduced with AAV-DJ showed at the top of the frame. (C) Trypan blue stained MSC were counted at a magnification of ×100 in triplicate. Data are expressed as mean±s.d.