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. 2021 Feb 5;7(5):372–390. doi: 10.1159/000512986

Fig. 2.

Fig. 2

Smad3 KO attenuated cisplatin-induced renal inflammation and NOX4-dependent oxidative stress in vivo. a Immunohistochemistry of F4/80+ and TNF-α. Smad3 KO reduced inflammatory response, including recruitment of macrophages and secretion of TNF-α, as the arrows shown in pictures. b Real-time PCR for inflammatory indicators in mice. Smad3 KO reduced mRNA levels of TNF-α, IL-6, and MCP-1 compared with those in S3WT mice. c Western blot analysis of NOX4. d Western blot analysis of NDUFA 9. Data represent mean ± SEM for 6–8 mice. *p < 0.05, **p < 0.01, ***p < 0.001 versus normal; #p < 0.05, ##p < 0.01, ###p < 0.001 versus S3FF + cisplatin group. Magnification: ×20. KO, knockout; WT, wild type.