Fig. 3. Protein tyrosine phosphorylation changes in spermatozoa obtained by swim-up (SU) and density gradient centrifugation (DGC) (n=7). Changes in SU- and DGC-sperm protein tyrosine phosphorylation (pY) were evaluated by Western blot. After pY detection, membranes were re-probed for β-tubulin (tub) to be used as loading control. (A) Representative Western blots of pY and tub. (B) Densitometric analysis of normalized intensities (pY/tub). ^*p<0.05 vs. SU at same incubation time.