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. Author manuscript; available in PMC: 2021 Dec 24.
Published in final edited form as: ACS Sens. 2020 Dec 11;5(12):3879–3891. doi: 10.1021/acssensors.0c01231

Figure 2: Targeting domains derived from vesicular cargo proteins fused with ZapCY1 accumulate in vesicular puncta in HeLa cells and MIN6 cells.

Figure 2:

(A) Representative images of HeLa cells (top row) and MIN6 cells (bottom row) expressing VAMP2-ZapCY1, CgA-ZapCY1 and NPY-ZapCY1 that were collected using a spinning disk microscope. The images shown are an overlay of the CFP channel (green) and YFP channel (magenta). Scale bar = 10 μm. (B) Dot plot of CFP puncta detected per HeLa cell, normalized to cell area (μm2). Three independent experiments were performed per construct, and the average ± standard deviation is shown for n=15 cells (VAMP2), n=19 cells (CgA) and n=16 cells (NPY). Two outliers were identified in the VAMP2 group using the ROUT method (Q=1%) and removed from further analysis. (C) Dot plot of CFP puncta detected per MIN6 cell, normalized to cell area (μm2). Three independent experiments were performed per construct, and the average ± standard deviation is shown for n=22 cells (VAMP2), n=31 cells (CgA) and n=23 cells (NPY). Four outliers were identified in the VAMP2 group using the ROUT method (Q=1%) and removed from further analysis. Statistical analysis for B and C was performed using a One-Way ANOVA test with post hoc Tukey (**** P < 0.0001, ** P < 0.01 compared with VAMP2).