Table 2.
Overview for immobilisation of peroxygenases.
| Enzyme | Carrier (Interaction) | Immobilisation yield | Recovered activity | Reaction catalysed |
Remarks | Ref. |
|---|---|---|---|---|---|---|
| Carrier-bound approaches | ||||||
| CfuCPO | Aminopropyl glass (covalent) |
91% | TMPD oxidation | Improved storage stability | (Kadima and Pickard, 1990) | |
| CfuCPO | Methacrylate polymer (covalent) | n.d. | 83% | Monochloro-dimedon chlorination | Storage and thermal stability increased | (Bayramoglu et al., 2011) |
| CfuCPO | Mesoporous silica (covalent) |
11% | n.d. | Azo dye oxidation | Increased temperature stability and kcat/KM | (Guerrero et al., 2012) |
| CfuCPO | Mesoporous silica (adsorptive) |
80% | n.d. | Styrene oxidation | Increased stability against temperature and acetonitrile | (Águila et al., 2011) |
| CfuCPO | Magnetic beads (covalent) |
n.d. | 58% | Monochloro-dimedon chlorination | Improved thermal and storage stability | (Bayramoğlu et al., 2008) |
| CfuCPO | Chitosan (covalent) |
n.d. | n.d. | Monochloro-dimedon chlorination | Improved thermal and oxidative stability | (Zhang et al., 2009) |
| CfuCPO | Agarose (adsorptive) |
75% | 50% | Monochloro-dimedon chlorination | Increased stability against tert-butyl hydroperoxide | (Pešić et al., 2012) |
| CfuCPO | Agarose (covalent) |
94% | 55% | Monochloro-dimedon chlorination | (Pešić et al., 2012) | |
| CfuCPO | TiO2 nanotubes (adsorptive) |
35% | 26% | Styrene epoxidation | Increased kcat and operational stability | (Muñoz-Guerrero et al., 2015) |
| AaeUPO | Methacrylate polymers (covalent) |
n.d. | 4% | Ethylbenzene hydroxylation | Used in neat substrate | (Fernández-Fueyo et al., 2016) |
| AaeUPO | Methacrylate polymers (covalent) |
n.d. | n.d. | Photo-biocatalytic ethylbenzene hydroxylation | 8x increase of TON compared to free enzyme | (Zhang et al., 2018) |
| AaeUPO | Agarose (covalent) |
n.d. | 15% | Styrene epoxidation | Increased stability against acetonitrile, high temperature, high pH | (Molina-Espeja et al., 2019) |
| AaeUPO | Polyacryl (covalent) |
72.8% | 3% | Styrene epoxidation | Up to 360 mM product | (Rauch et al., 2019) |
| Carrier-free approaches | ||||||
| CfuCPO | Aggregation and cross linking | n.d. | 68% | Thioanisole oxidation | Higher tolerance against H2O2, temperature and pH | (Perez et al., 2009a) |
|
MroUPO/ AaeUPO |
PVA/PEG Gel (entrapment) |
n.d. | 86% | Diclofenac oxidation | 60 fold increase in TON, storage in cyclohexane increased activity | (Poraj-Kobielska et al., 2015) |
| AaeUPO | Calcium alginate entrapment | 81% | - | Hydroxylation of cyclohexane and cyclopentane | Entrapment enabled light driven solvent free alkane hydroxylation | (Hobisch et al., 2020) |
TMPD: N,N,N',N'-tetramethyl-p-phenylenediamine