FIG. 8.

TIMP-1 and TIMP-2 neutralizing antibodies and 786-0 RCC cell in vitro invasiveness. Invasion assays were performed in collagen type IV by the method described in the legend to Fig. 2. Heat-inactivated neutralizing rabbit anti-human TIMP-1 and TIMP-2 antibodies (or two different preimmune rabbit sera) were used at 5% (vol/vol) dilutions in the upper compartment. A greater number of invading cells per filter in the VHL-negative cells than in the VHL-positive cells in the presence of HGF/SF (20 ng/ml) and neutralizing TIMP-1 (Neut-T₁) alone or in combination with neutralizing TIMP-2 (Neut-T₂) was found (see Table 1). WT, VHL-positive 786-0 RCC cells (WT-7); Mut, VHL-negative 786-0 RCC cells (ARZ-2).