FIG. 9.

TIMP-1 and TIMP-2 and branching morphogenesis in VHL-positive and VHL-negative RCC cells. Branching morphogenesis assays on 786-0 RCC cells were performed as described in the legend to Fig. 1. TIMP-1 (6.4 μg/ml) or TIMP-2 (10 μg/ml) were included in both the gel and the supernatant. Control wells received equal volumes of PBS. The assay was performed for all wt and mut VHL cell lines (data shown for only VHL-negative 786-0 RCC cells). For any cell line, treatment of cells expressing wt VHL with TIMP-1 or TIMP-2 resulted in no response (data not shown). Each experiment was performed in quadruplicate, and the assays were repeated three times.