Table 3.
In silico sequence analysis of candidate antibodies for common liability motifs
| ID | Mouse strain | Liability (High) | Liability (Medium) | Liability (Low) |
|---|---|---|---|---|
| 15G11 | DivergimAb | DS (Isomerization, Heavy FR3); M (Oxidation, Heavy CDR3) | TS (Cleavage, Heavy FR3, Heavy FR4); TS (Cleavage, Light CDR1, Light FR1); NH (Deamidation, Light CDR3) | SN (Deamidation, Light CDR1, Light CDR3); TN (Deamidation, Light CDR2) |
| 18F4 | DivergimAb | DP (Cleavage, IGHG2B); NA (Deamidation, Heavy CDR3); NS (Deamidation, 3 * Heavy FR3); M (Oxidation, Heavy CDR3) | TS (Cleavage, Heavy CDR1, Heavy FR4); TS (Cleavage, Light CDR1, Light FR1); NH (Deamidation, Light CDR3) | TN (Deamidation, IGHV2-9-1); SN (Deamidation, Light CDR1, Light CDR3); TN (Deamidation, Light CDR2) |
| 1E5 | DiversimAb | DS (Isomerization, Heavy FR3); M (Oxidation, Heavy CDR3); NG (Deamidation, Light CDR1); M (Oxidation, Light CDR2) | TS (Cleavage, Heavy CDR1, 2 * Heavy FR3, Heavy FR4); TS (Cleavage, Light FR1); NP (Hydrolysis, Light FR1) | TN (Deamidation, IGHV1-55); SN (Deamidation, IGKV2-109, Light CDR1, Light FR1) |
| 1G3 | DiversimAb | NS (Deamidation, 3 * Heavy FR3, IGHG1) | TS (Cleavage, Heavy CDR1); TS (Cleavage, Light CDR1, Light FR1); NH (Deamidation, Light CDR3) | TN (Deamidation, IGHG1, IGHV2-9-1); SN (Deamidation, Light CDR1, Light CDR3); TN (Deamidation, Light CDR2) |
| 21C3 | DivergimAb | NS (Deamidation, 3 * Heavy FR3) | TS (Cleavage, Heavy CDR1); TS (Cleavage, Light CDR1, Light FR1) | TN (Deamidation, IGHV2-9-1); SN (Deamidation, Light CDR1, Light CDR3); TN (Deamidation, Light CDR2) |
| 22D9 | DivergimAb | DS (Isomerization, Heavy FR3); NS (Deamidation, Light CDR1); DG (Isomerization, Light FR3) | TS (Cleavage, Heavy FR1, Heavy FR3); NH (Deamidation, IGHG1); NP (Hydrolysis, Heavy CDR2) | TN (Deamidation, Heavy CDR1, IGHV1-54) |
| 23D11 | DivergimAb | NG (Deamidation, Heavy CDR2); DG (Isomerization, Heavy CDR3); DS (Isomerization, Heavy FR3); M (Oxidation, Heavy CDR3); NS (Deamidation, Light CDR3) | TS (Cleavage, Heavy FR1, Heavy FR3, Heavy FR4, IGHV1S113); NP (Hydrolysis, Heavy CDR2); TS (Cleavage, Light FR1) | SN (Deamidation, Light FR3); TN (Deamidation, Light CDR1) |
| 26E2 | DivergimAb | DP (Cleavage, Heavy CDR2); NG (Deamidation, Heavy CDR2); DS (Isomerization, IGHG2A;IGHG2C) | TS (Cleavage, 2 * Heavy FR3, IGHG2A;IGHG2C) | SN (Deamidation, Heavy FR3); SN (Deamidation, Light FR3) |
| 29F7 | DivergimAb | NG (Deamidation, Heavy FR3); DG (Isomerization, Heavy CDR2); DS (Isomerization, Heavy FR3, IGHG2C); NS (Deamidation, Light CDR3) | TS (Cleavage, Heavy FR3); TS (Cleavage, Light FR1) | TN (Deamidation, IGHV1-82); SN (Deamidation, Light FR3); TN (Deamidation, Light CDR1) |
| 3B3 | DiversimAb | NA (Deamidation, Heavy FR3); DG (Isomerization, Heavy CDR3); DP (Cleavage, Light CDR3, Light FR3); NS (Deamidation, Light CDR1); DS (Isomerization, Light CDR1) | SN (Deamidation, IGKV3-10) | |
| 3F2 | DiversimAb | DP (Cleavage, Heavy CDR2); NG (Deamidation, Heavy CDR2); NS (Deamidation, Heavy FR3); | TS (Cleavage, Heavy FR3); TS (Cleavage, Light CDR1, Light FR1); NH (Deamidation, Light CDR3) | SN (Deamidation, Heavy CDR3, Heavy FR3); SN (Deamidation, Light CDR1, Light CDR3); TN (Deamidation, Light CDR2) |
| D59047-11955 | DivergimAb | NS (Deamidation, 3 * Heavy FR3) | TS (Cleavage, Heavy CDR1); TS (Cleavage, Light CDR1, Light FR1) | TN (Deamidation, IGHV2-9-1); SN (Deamidation, Light CDR1, Light CDR3); TN (Deamidation, Light CDR2) |
| D70678-12637-S1 | Alloy | NA (Deamidation, Heavy FR3); NS (Deamidation, 2 * Heavy FR3); DS (Isomerization, Heavy FR3) | TS (Cleavage, Heavy FR1) | KN (Deamidation, Heavy FR3); SN (Deamidation, Heavy CDR2) |
| D70678-12799-S1 | Alloy | NS (Deamidation, Heavy CDR2); DG (Isomerization, Light CDR1); M (Oxidation, Light CDR3) | TS (Cleavage, Heavy FR3); NP (Hydrolysis, Heavy CDR2) | TN (Deamidation, IGHV1-2); SN (Deamidation, IGKV2-24) |
| D70678-13531-S1 | Alloy | NS (Deamidation, Heavy FR3); M (Oxidation, Heavy CDR3); 2 C (Extra Cysteine, Light FR1); DG (Isomerization, Light CDR1); DS (Isomerization, Light FR3); M (Oxidation, Light CDR3) | TS (Cleavage, Heavy FR3); NP (Hydrolysis, Heavy FR3) | TN (Deamidation, Heavy FR3) |
| D70678-13576-S1 | Alloy | NS (Deamidation, Heavy FR3); M (Oxidation, Heavy CDR3); 2 C (Extra Cysteine, Light FR1); DG (Isomerization, Light CDR1); DS (Isomerization, Light FR3); M (Oxidation, Light CDR3); DS (Isomerization, Light FR1) | TS (Cleavage, Heavy FR3); NP (Hydrolysis, Heavy FR3) | SN (Deamidation, Heavy CDR1); TN (Deamidation, Heavy FR3); KN (Deamidation, Light CDR1); SN (Deamidation, Light CDR1) |
| D70678-14004-S2 | Alloy | DP (Cleavage, Heavy CDR3); NG (Deamidation, Heavy CDR2); | TS (Cleavage, 2 * Heavy FR3) | TN (Deamidation, IGHV1-18) |
| D70678-14027-S2 | Alloy | NS (Deamidation, Heavy FR3); DG (Isomerization, Heavy CDR2); DS (Isomerization, Heavy FR3); | KN (Deamidation, Heavy FR3); SN (Deamidation, IGKV1-33;IGKV1D-33) | |
| D70678-2155-S1 | Alloy | NA (Deamidation, Heavy CDR1); NS (Deamidation, Heavy FR3); DG (Isomerization, Heavy CDR2); DS (Isomerization, Heavy FR3); | KN (Deamidation, Heavy FR3); SN (Deamidation, Heavy CDR1) | |
| D70678-2743-S1 | Alloy | NS (Deamidation, Heavy FR3); M (Oxidation, Heavy CDR3); DG (Isomerization, Light CDR1); DS (Isomerization, Light FR3); M (Oxidation, Light CDR3) | TS (Cleavage, Heavy FR3); NP (Hydrolysis, Heavy FR3) | TN (Deamidation, Heavy FR3); SN (Deamidation, IGKV2-30) |
| D70678-5521-S2 | Alloy | DS (Isomerization, Heavy CDR2, Heavy FR3) | NH (Deamidation, Light CDR1) | KN (Deamidation, Heavy FR3); SN (Deamidation, IGKV1-33;IGKV1D-33) |