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. 2021 Sep 16;19:94. doi: 10.1186/s12964-021-00770-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — IL-6 signalling results in a dose-dependent heterogeneous STAT3 activation. a, b MEF wt cells c, d MEF ΔEx3 cells e, f MEF ΔEx3 + SHP2 cells were stimulated with increasing amounts of Hy-IL-6 for 15 min. STAT3 expression and phosphorylation were evaluated by intracellular multiplex flow cytometry using specific fluorescent antibodies against STAT3 (A, C, E) and STAT3-Y705 phosphorylation (B, D, F). Representative histograms of n = 3 independent experiments per cell line are shown. Maximal mean fluorescence was normalised to 100%. Log₁₀ normalised data points are given as boxplots with median and IQR (box). For better visualisation, only ~ 5% of all data points (n = 1000) per concentration from 3 independent experiments were randomly selected for display

Fig. 2 — IL-6 signalling results in a dose-dependent heterogeneous STAT3 activation. a, b MEF wt cells c, d MEF ΔEx3 cells e, f MEF ΔEx3 + SHP2 cells were stimulated with increasing amounts of Hy-IL-6 for 15 min. STAT3 expression and phosphorylation were evaluated by intracellular multiplex flow cytometry using specific fluorescent antibodies against STAT3 (A, C, E) and STAT3-Y705 phosphorylation (B, D, F). Representative histograms of n = 3 independent experiments per cell line are shown. Maximal mean fluorescence was normalised to 100%. Log₁₀ normalised data points are given as boxplots with median and IQR (box). For better visualisation, only ~ 5% of all data points (n = 1000) per concentration from 3 independent experiments were randomly selected for display