Table 2. Linear Regression Range, LOD and LOQ, Recovery, and Precision of the Developed HPLC-PDA Method at Different Concentrations of Picrocrocin, trans-4-GG-Crocin, trans-3-Gg-Crocin, cis-4-GG-Crocin, trans-2-gg-Crocin, trans-Crocetin, and Safranal in HPLC-PDA Single-Laboratory-Based Validation.
| analyte | Rt | wavelength (mm) | range (μg/mL) | linear regression equationa | R2 | LOD (μg/mL) | LOQ (μg/mL) | average recoveryb (±SD) | intermediate precision (%, RSD) |
|---|---|---|---|---|---|---|---|---|---|
| picrocrocin | 7.435 | 250 | 0.78–100 | y = 15392x – 955.9 | 1.000 | 0.178 | 0.844 | 90.36 ± 0.55 | 0.684 |
| trans-4-GG-crocin | 8.555 | 440 | 0.78–100 | y = 58363x + 20042 | 0.999 | 0.277 | 0.839 | 82.49 ± 2.48 | 0.506 |
| trans-3-Gg-crocin | 8.971 | 440 | 0.31–40 | y = 82239x – 658.89 | 0.999 | 0.095 | 0.288 | 86.55 ± 2.15 | 1.674 |
| cis-4-GG-crocin | 9.783 | 440 | 3.12–400 | y = 925.81x + 2302 | 0.999 | 0.978 | 2.964 | 88.65 ± 1.95 | 0.544 |
| trans-2-gg-crocin | 10.261 | 440 | 3.12–400 | y = 1799.9x – 295.52 | 0.999 | 0.877 | 2.659 | 90.78 ± 2.15 | 0.525 |
| trans-crocetin | 13.419 | 440 | 0.16–20 | y = 73529x – 2315.7 | 1.000 | 0.048 | 0.146 | 90.96 ± 2.63 | 0.577 |
| safranal | 14.059 | 320 | 0.16–20 | y = 33447x + 271.5 | 1.000 | 0.040 | 0.122 | 92.96 ± 0.91 | 0.557 |
a
Linear regression replications (n = 8 × 5).
b
% recovery (n = 3 × 3) and precision (n = 6 × 3).