Table 3. Linearity Results and Precision of Area and Retention Time of Standard Solution by HPLC-PDA Methodologya.
| peak response
(AUC) |
retention time (Rt) |
|||||
|---|---|---|---|---|---|---|
| compound | *concentration | mean peak area | **RSD | R2 | mean (min) | **RSD |
| picrocrocin | 0.78 | 13,119 | 1.244 | 1.0000 | 7.44 | 0.066 |
| 1.56 | 25,334 | 0.221 | 7.45 | 0.132 | ||
| 3.13 | 48,211 | 0.648 | 7.45 | 0.081 | ||
| 6.25 | 94,756 | 0.931 | 7.47 | 0.073 | ||
| 12.5 | 186,033 | 0.525 | 7.46 | 0.060 | ||
| 25 | 387,530 | 1.026 | 7.47 | 0.154 | ||
| 50 | 762,967 | 0.242 | 7.47 | 0.060 | ||
| 100 | 1,540,760 | 0.142 | 7.46 | 0.100 | ||
| trans-4-GG crocin | 0.78 | 46,612 | 0.342 | 0.9999 | 8.56 | 0.052 |
| 1.56 | 94,230 | 0.515 | 8.56 | 0.057 | ||
| 3.13 | 185,125 | 0.104 | 8.56 | 0.064 | ||
| 6.25 | 370,392 | 1.038 | 8.56 | 0.000 | ||
| 12.5 | 737,008 | 0.592 | 8.57 | 0.000 | ||
| 25 | 1,542,668 | 0.879 | 8.57 | 0.107 | ||
| 50 | 2,996,811 | 0.230 | 8.57 | 0.057 | ||
| 100 | 5,814,605 | 0.167 | 8.57 | 0.057 | ||
| trans-3-Gg crocin | 0.31 | 25,326 | 0.149 | 0.9999 | 8.97 | 0.061 |
| 0.63 | 51,050 | 0.674 | 8.97 | 0.061 | ||
| 1.25 | 100,145 | 0.078 | 8.98 | 0.061 | ||
| 2.5 | 200,421 | 1.042 | 8.98 | 0.055 | ||
| 5 | 399,730 | 0.592 | 8.98 | 0.067 | ||
| 10 | 840,876 | 0.881 | 8.98 | 0.109 | ||
| 20 | 1,645,852 | 0.223 | 8.99 | 0.075 | ||
| 40 | 3,284,919 | 0.090 | 8.98 | 0.067 | ||
| cis-4-GG crocin | 3.12 | 3517 | 1.363 | 0.9999 | 9.78 | 0.056 |
| 6.25 | 6349 | 0.614 | 9.76 | 0.000 | ||
| 12.5 | 14,124 | 0.848 | 9.76 | 0.050 | ||
| 25 | 25,724 | 0.959 | 9.76 | 0.062 | ||
| 50 | 49,984 | 0.700 | 9.76 | 0.070 | ||
| 100 | 97,005 | 0.454 | 9.76 | 0.000 | ||
| 200 | 187,596 | 0.276 | 9.76 | 0.062 | ||
| 400 | 371,869 | 0.117 | 9.77 | 0.057 | ||
| trans-2-gg crocin | 3.12 | 6203 | 0.610 | 1.0000 | 10.25 | 0.059 |
| 6.25 | 11,923 | 1.193 | 10.23 | 0.089 | ||
| 12.5 | 23,244 | 0.658 | 10.23 | 0.069 | ||
| 25 | 45,552 | 0.466 | 10.23 | 0.049 | ||
| 50 | 90,122 | 0.675 | 10.23 | 0.079 | ||
| 100 | 177,148 | 0.144 | 10.23 | 0.099 | ||
| 200 | 356,131 | 0.563 | 10.23 | 0.059 | ||
| 400 | 722,238 | 0.206 | 10.23 | 0.099 | ||
| trans-crocetin | 0.16 | 11,270 | 0.454 | 1.0000 | 13.42 | 0.033 |
| 0.31 | 22,426 | 0.774 | 13.42 | 0.033 | ||
| 0.63 | 44,264 | 0.218 | 13.42 | 0.041 | ||
| 1.25 | 88,390 | 1.103 | 13.43 | 0.037 | ||
| 2.5 | 176,108 | 0.601 | 13.43 | 0.037 | ||
| 5 | 370,557 | 0.968 | 13.43 | 0.045 | ||
| 10 | 728,893 | 0.221 | 13.44 | 0.000 | ||
| 20 | 1,469,695 | 0.071 | 13.43 | 0.045 | ||
| safranal | 0.16 | 6250 | 0.964 | 0.9996 | 14.05 | 0.035 |
| 0.31 | 11,433 | 1.114 | 14.05 | 0.053 | ||
| 0.63 | 21,535 | 0.286 | 14.06 | 0.063 | ||
| 1.25 | 41,788 | 1.005 | 14.06 | 0.067 | ||
| 2.5 | 81,578 | 0.567 | 14.07 | 0.035 | ||
| 5 | 169,766 | 0.989 | 14.07 | 0.043 | ||
| 10 | 332,776 | 0.055 | 14.07 | 0.043 | ||
| 20 | 669,921 | 0.042 | 14.06 | 0.053 | ||
a
*μg/mL, **relative standard deviation.