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. 2021 Sep 16;18:206. doi: 10.1186/s12974-021-02263-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Evaluation of the effects of 1,25(OH)₂D₃ treatment on PERG, FERG, and IOP. A Representative waveforms of PERG and FERG at baseline and at the end of treatment. Each waveform, deriving from PERG and FERG recordings, was analysed for the peak-to-trough amplitude. B (PERG) and C (FERG) report the plot of amplitude values vs. time of treatment, as regards control DBA/2J and 1,25(OH)₂D₃-treated mice. PERG was recorded at baseline and from the 2nd to the 5th week of treatment; FERG was recorded at baseline and from the 3rd week of treatment. D IOP values normalized to baseline data, over time. Data were analysed using the GEE statistics to test the effects of time and of treatment; n = 30 independent records per experimental group, two eyes per mouse, 15 mice per group.