Table 2.
Platelet function parameters in survivors and non-survivors on day 1 of ECMO. Data are presented as mean ± SEM, n = 15 survivors, n = 15 non-survivors; p-values were calculated by an unpaired Student’s t-test. p-values < 0.05 are highlighted in bold. Parameters were recorded as described in the Methods section. ECMO, extracorporeal membrane oxygenation; MFI, mean fluorescence intensity; PLA, platelet leukocyte aggregates
| Parameter | Survivors | Non-Survivors | p-value |
|---|---|---|---|
| Baseline CD62P expression (%) | 16.7 ± 2.4 | 16.6 ± 2.7 | > 0.99 |
| CD62P + TRAP (%) | 74.9 ± 3.9 | 68.5 ± 4.9 | 0.32 |
| CD62P + ADP (%) | 66.7 ± 6.3 | 51.3 ± 5.5 | 0.07 |
| Baseline CD63 expression (%) | 12.4 ± 2.0 | 24.0 ± 2.8 | 0.003 |
| CD63 + TRAP (%) | 47.3 ± 5.4 | 50.4 ± 3.2 | 0.63 |
| CD63 + ADP (%) | 31.1 ± 4.6 | 32.8 ± 3.4 | 0.77 |
| Baseline PAC-1 binding (%) | 0.34 ± 0.08 | 0.27 ± 0.06 | 0.47 |
| PAC-1 + TRAP (%) | 13.8 ± 3.3 | 5.4 ± 2.1 | 0.04 |
| PAC-1 + ADP (%) | 33.7 ± 6.5 | 12.3 ± 4.0 | 0.009 |
| GPVI (MFI) | 2,271.0 ± 115.6 | 2,248.0 ± 162.0 | 0.91 |
| GPIbα (MFI) | 11,440 ± 797.9 | 12,955 ± 1,006 | 0.25 |
| Baseline PLA (%) | 2.9 ± 0.8 | 5.8 ± 1.4 | 0.07 |
| Stimulated PLA (%) | 30.4 ± 3.6 | 28.0 ± 4.0 | 0.66 |
| Mepacrine Assay - % of baseline MFI after 20 min thrombin stimulation | 78.1 ± 2.7 | 81.4 ± 2.7 | 0.39 |
| Platelet count x103/µl | 142.2 ± 15.8 | 116.5 ± 30.6 | 0.46 |