Fig. 5.

Exploration of potential mechanisms of NAD⁺ effects in CCH model. A RNA-seq results showing hierarchical clustering of co-regulated DEGs. B Volcano plotting of DEGs. C The significantly changed genes that were analyzed by KEGG enrichment. D List of enriched GO term on biological process, cellular component, and molecular function. E Western blot analysis of Sirt1, PGC-1α in hippocampus. Data are expressed as mean ± SEM (n = 3). Data are expressed as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, vs. CCH