Figure 3 .

Sevoflurane-induced alterations in TF distribution in sperm of F1 animals. (A) Pearson correlation heatmaps of read coverage at differential THSSs when comparing sperm ATAC-seq from control, 7-day–exposed, and 1-day–exposed F1 animals. THSSs are defined as regions ±75 bp from the summits. (B) k-means clustering analysis of differential THSSs (n = 1211) using subnucleosome-size reads from ATAC-seq experiments. Fold change of pair-wise comparisons (shown on the left) of 7-day–exposed F1 vs control and 1-day–exposed F1 vs control was used in k-means clustering. The corresponding normalized subnucleosomal reads are shown on the right for control, 7-day–exposed F1, and 1-day–exposed F1 datasets. (C) Genome-wide distribution of THSSs present in the different clusters shown in panel (B); promoters were defined as TSS-0.5 kb. (D) TF motif archetype enrichment analysis at differentially accessible clusters from panel (B) showing increased accessibility (clusters 3, 4, 5, and 6; top) and lost accessibility (clusters 1 and 2; bottom). TF motif archetype clusters with P-value less than 0.001 based on Monte Carlo permutation tests simulated genome-wide were labeled with a pink dot. TF motif archetypes with P-value < 0.001 in the permutation tests simulated within the pool of ENCODE’s candidate cis-regulatory elements (cCREs) were marked in green.