Fig. 2.

Validation of bisulfite amplicon sequencing (BSAS) method for assaying MGMT promoter methylation level. (A) Association between methylation level at CpG site +174 bp from TSS assayed by BSAS and by Infinium Array across samples. (B) Association between methylation level at each CpG site assayed by BSAS and MGMT mRNA level across samples. Pearson correlations and P-values adjusted for multiple hypothesis testing are shown for each CpG site labeled by distance from TSS. Sequence annotations are shown for regions assayed by methylation-specific PCR (MSP), an enhancer, and regions for which associations between methylation and transcription have previously been shown (hotspot, DMR, concordant region).