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. 2021 Sep 2;11:702258. doi: 10.3389/fonc.2021.702258

Figure 4.

Figure 4

Cell-culture-based assessment. The effect of miR-122-5p and miR-142-5p on cell proliferation (A–D), colony-forming activity (E–H), and cell cycle distribution (I-P) in rectal cancer cell lines. (A, B) - A significant decrease in cell proliferation of SW1463 cells after the miR-122-5p transfection (A) was observed after both 24h and 48h (24h: without 5-FU: p<0.001, with 5-FU: p=0.0003; 48h: without 5-FU: p=0.2, with 5-FU: p=0.008). Same trend was recorded for HRA16 [(B), for 24 h: p=0.05, for 48 h: p= 0.04]. (C, D) miR-142-5p transfection had a moderate impact on SW1463 proliferation [(C), p=0.007] but no effect on HRA16 proliferation (D). (E, F–H) Both HRA16 and SW1463 transfected with miR-122-5p and miR-142-5p mimics formed significantly fewer colonies than those transfected with control oligonucleotide (miR-122-5p: SW1463 - no significant results, HRA16 – p=0.05; miR-142-5p: SW1463 – p=0.02, HRA16 – p=0.001). The same effect was also observed after the 5-FU administration (miR-122-5p + 5-FU: SW1463 – no significant results, HRA16 – p=0.01; miR-142-5p + 5FU: SW1463 – p=0.0008, HRA16 – p=0.005). (I–P) No significant change in cell cycle progression after miR-122-5p and miR-142-5p transfection was observed. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.