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. 2021 Sep 2;12:660887. doi: 10.3389/fmicb.2021.660887

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Copyright © 2021 Eckroat, Greguske and Hunnicutt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of media concentration on biofilm formation. The planktonic growth (orange-striped bars) and biofilm formation (blue-dotted bars) of Flavobacterium johnsoniae wild type grown for 24h in a series of dilutions of casitone-yeast extract (CYE) media. Results are reported as relative optical density (OD) values (averages of at least four replicates; normalized to highest average in dataset). Error bars indicate 95% CI. Different letters above the error bars represent different significance groups among growth and biofilm measurements (one-way ANOVA followed by Tukey test, value of p=0.05).