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. 2021 Sep 2;12:660887. doi: 10.3389/fmicb.2021.660887

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Copyright © 2021 Eckroat, Greguske and Hunnicutt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of motility and type IX secretion system (T9SS) gene deletions on biofilm formation. Biofilm formation was determined for strains with knockouts in genes linked to gliding motility (gldI, gldJ), the T9SS (gldJ, gldNO), and colony spreading (sprB). Knockouts (blue-dotted bars) and complemented knockouts (yellow-dashed bars) of F. johnsoniae were gldI and gldI^c: UW102-41 and CJ1171; gldJ and gldJ^c: UW102-55 and CJ1111; gldNO and gldNO^c: CJ1631A and CJ1640; and sprB and sprB^c: CJ1922 and CJ2405. Results are reported as relative OD values (averages of at least four replicates; normalized to the wild type). Error bars indicate 95% CI. Different letters above the error bars represent different significance groups (one-way ANOVA followed by Tukey test, value of p=0.05).