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. 2021 Sep 2;12:660887. doi: 10.3389/fmicb.2021.660887

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Copyright © 2021 Eckroat, Greguske and Hunnicutt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biofilm formation by non-motile, T9SS-competent mutants and motile, T9SS-deficient mutants. Biofilm formation was determined for strains with knockouts in genes resulting in the loss of T9SS function (porV), motility (gldJ_t548 and gldJ_t553), and both T9SS and gliding motility (gldJ). Knockouts (blue-dotted bars) and complemented knockouts (yellow-dashed bars) of F. johnsoniae were porV and porV^c: CJ2130 and CJ2169; gldJ and gldJ^c: UW102-55 and CJ1111; gldJ_t548 and gldJ_t548^c: CJ2386 and CJ2405; and gldJ_t553 and gldJ_t553^c: CJ2443 and CJ2484. Results are reported as relative OD values (averages of at least four replicates; normalized to the wild type). Error bars indicate 95% CI. Different letters above the error bars represent different significance groups (one-way ANOVA followed by Tukey test, value of p=0.05).