TABLE 2.
Increased sporulation and spore viability in strains with mutations in the exonuclease motif of Xrn1p compared to the deletion strain
| Relevant genotype | % Sporulationa | % Spore viabilityb |
|---|---|---|
| wt* | 79.4 ± 4.6 | 96.8 ± 2.1 |
| xrn1Δ | 7.8 ± 1.6 | 57.5 ± 9.5 |
| xrn1-D206A | 26.4 ± 2.7 | 87.7 ± 8.3 |
| xrn1-D208A | 39.7 ± 4.7 | 88.4 ± 4.8 |
| xrn1-D206A,D208A | 24.7 ± 7.2 | 84.3 ± 3.1 |
a
Sporulation was quantified in isogenic SK-1 strains WDHY549 × WDHY550 (wt* denotes the exact isogenic control [see Materials and Methods]), WDHY143 × WDHY551 (xrn1Δ), WDHY489 × WDHY552 (xrn1-D206A), WDHY490 × WDHY553 (xrn1-D208A), and WDHY491 × WDHY554 (xrn1-D206A,D208A), counting at least 200 cells for each determination. Numbers are means ± standard deviations from five independent sporulation experiments.
b
The viability of spores derived from the same strains was determined by dissecting a total of 204 tetrads. Means ± standard deviations for the five independent strains are shown.