Figure 2.

Deletions within the CR change the condensation behavior of TDP-43 in cells

(A) Maximum z-projection images of HEK293 Flp-In cell lines expressing doxycycline (dox)-inducible GFP-TDP-43 variants.

(B) Heterogeneous expression levels of GFP-TDP-43 WT were induced with a dox time course (4–72 h) in HEK293 Flp-In cells, n (cells) = 118. Foci counts in each segmented nucleus are plotted against the total nuclear fluorescence of individual GFP-TDP-43 WT cells.

(C) As in (B), the fractional nuclear area occupied by the sum of all foci in each nucleus plotted against the mean nuclear fluorescence.

(D) As in (B), the relationship between the mean foci fraction fluorescence intensity and mean nuclear fluorescence.

(E) Quantification of foci counts in each segmented nucleus. Mean ± 95% confidence interval (CI) is shown. n (cells): WT = 21, 274del319 = 20, 316del346 = 22, 320del366 = 20, 367del414 = 23, 320del414 = 23.

(F) FRAP experiments on HEK293 cell lines. The fluorescence recovery curve was obtained by bleaching a spot of predefined size in the nucleoplasm. Mean ± 95% CI is shown for 34 cells for all cell lines.

(G) Rate constant of fluorescence recovery. Mean ± 95% CI is shown. n (cells) = 34 for all cell lines.

Significance for (E) and (G) was tested with Kruskal-Wallis test followed by Dunn’s multiple comparisons test. Reported adjusted (adj.) p values are for the individual comparisons (^∗p adj. < 0.05, ^∗∗∗p adj. < 0.001, and ^∗∗∗∗p adj. < 0.0001).