A whole-body serial block-face scanning electron microscopy dataset
(A) The SBEM dataset can be observed in all orientations (e.g., transversal plane in top row or the horizontal plane in bottom row; scale bar: 50 μm).
(B–E) Fine ultrastructure at native resolution (10 nm pixel-size x/y; scale bars: 2 μm).
(B) Epithelial cell, interfacing cuticle, and underlying muscle. Bundles of cytoskeletal filaments (arrowhead) form part of the attachment complex (inset).
(C) The adult eye forms a pigment cup composed of pigment cells (PiCs) and rhabdomeric photoreceptors (rPRCs), which extend a distal segment of microvillar projections (mi) for light detection. In the center of the pigment cup is the vitreous body (vb).
(D) Longitudinal muscle fibers are cut transversally, displaying cross-sections of the sarcomere as well as of the sarcoplasmic reticulum that contacts the plasma membrane (inset).
(E) Cross-section of the distal part of the nephridia, highlighting the autocell junction (arrow). The lumen houses a bundle of motile cilia (with 9+2 microtubules, inset) contributed by each nephridial cell.
(B)–(E) are snapshots that can be retrieved via the PlatyBrowser “Bookmark” function. See also Figure S1.