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. 2021 Sep 2;184(18):4819–4837.e22. doi: 10.1016/j.cell.2021.07.017

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Correlation of gene expression with morphologically defined tissues

(A) UMAP of all cells based on the expression of 201 genes. Points are colored by gene expression clusters (c0–c14). Gray rectangle, part of the UMAP shown in (D) and (E).

(B) Main body parts in 6-dpf Platynereis with matching colors between animal regions and UMAP.

(C) Gene clusters mapped onto an EM section.

(D) Comparison of anatomically (top) and genetically (bottom) defined units mapped on transversal (left) and horizontal (middle) head section and UMAP (right).

(E) F1 specificity score for mushroom bodies (MBs) and the 10 top scoring genes and clusters (see STAR Methods). The MB, cluster c2, and the expression of 12 genes mapped onto the head region of the UMAP are shown. See Figure S4 for specificity of the remaining ganglionic nuclei (GN).

For (C) and the EM overlays of (D), bookmarks are available in the PlatyBrowser. AEs, adult eyes; AG, antennal GN; CG, cirral GN; CpG, circumpalpal GN; DG, dorsal GN; FG, frontal GN; MBs, mushroom bodies; PG, palpal GN; VMG, ventro-medial GN. See also Figure S4.