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AAV5-mediated ChR2 is expressed in SPNs of D1/A2a-cre mice. A–D, Images of striatal neuropil to show localization of ChR2. A, Cre-dependent expression of ChR2 in cell membranes (arrowheads) in the striatum of A2a-cre mice. B, Spiny neurons labeled with DARPP-32. C, Cell nuclei stained with DAPI. D, Overlay showing DARP-32-positive neurons were also positive for ChR2 (arrowheads). Scale bar: 10 µm.
