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. Author manuscript; available in PMC: 2021 Sep 16.
Published in final edited form as: Cancer Res. 2020 Aug 28;81(2):371–383. doi: 10.1158/0008-5472.CAN-20-0571

Figure 1. Anchorage-independent growth of BrCa cells is abrogated by antiestrogens and rescued by BMSCs during drug exposure.

Figure 1.

a) MCF7 3D monocultures treated with DMSO, fulvestrant (300nM; 7 days) and 7 days after fulvestrant washout; H&E, magn x400. b) Fulvestrant-treated MCF7 2D and 3D cultures (Annexin V-based apoptosis assay; bioluminescence readout). c) Two-photon excited fluorescence imaging of fulvestrant (24h; 300nM)- vs. DMSO-treated MCF7 spheroids. d) Redox ratio slope from the peripheral cellular layers towards the center of MCF7 spheroids and degree of mitochondrial clustering, in monocultures or cocultures with BMSCs (fulvestrant 300nM 24hrs). e) Effect of N-Acetyl Cysteine on fulvestrant sensitivity in MCF7 2D and 3D cultures; 6 days. f) MCF7 spheroids in monocultures or cocultures with BMSCs, +/− fulvestrant (300nM; 7 days); H&E, magn. x200.