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. Author manuscript; available in PMC: 2021 Sep 16.
Published in final edited form as: Cancer Res. 2020 Aug 28;81(2):371–383. doi: 10.1158/0008-5472.CAN-20-0571

Figure 3. BMSCs attenuate estrogen-induced gene expression in BrCa spheroids independently of preservation or loss of ER expression.

Figure 3.

a) Transcriptional changes in BrCa 3D spheroids in cocultures (vs. respective monocultures; genes with significant changes in all cell lines are shown). b-c) Estrogen-responsive gene set in in MCF7 (b) and ZR75–1 (c) spheroids cocultured with BMSCs (vs. respective monocultures). d) ER transcript levels in BrCa spheroid cultures +/− BMSCs. e-f) ER immunostaining of MCF7 (e) or ZR75–1 (f) spheroid cultures +/− BMScs cells, +/− 4-OHT (300nM; 7 days), x200. (8 day cultures in panels a-d).