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. Author manuscript; available in PMC: 2021 Sep 16.
Published in final edited form as: Cell Rep. 2021 Sep 7;36(10):109670. doi: 10.1016/j.celrep.2021.109670

Figure 2. Precocious LIN-46 expression causes precocious cell-fate transitions in hypodermal seam cell lineage.

Figure 2.

(A) Number of seam cells observed in young adults (n = 20) of wild-type, lin-28(lf), three lin-46(gf) mutants (ma461, ma467, and ma472), and lin-28(lf); lin-46(ma467gf) double mutants. The Student’s t test was used to calculate statistical significance: Not significant (n.s.) p > 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001. The vertical bars show the average seam cell numbers observed in 20 animals.

(B) Representative (n = 20) DIC and fluorescent images (col-19p::gfp (maIs105)) of L4- and adult-stage animals. Scale bar: 50 μm.

(C) DIC images of a lin-46(ma461gf) larva showing the (precocious) adult-specific cuticle structure called alae (precocious alae in 55% of n = 20) on the cuticle of a larva at the L4 stage indicted by the developmental stage of the gonad. Scale bar: 25 μm and 5 μm (inset).

See also Table S2.