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. Author manuscript; available in PMC: 2021 Sep 16.
Published in final edited form as: Glia. 2020 Dec 23;69(5):1170–1183. doi: 10.1002/glia.23956

Figure 7. The laforin malin complex interacts with α-arrestin 1 and promotes its ubiquitination.

Figure 7.

(A) HEK293 cells were co-transfected with the plasmids expressing HA-Laforin, Flag-Malin, and GFP-tagged α-arrestin1 (ARRDC1-GFP) or GFP as control. Cells were lysed and 1.5 mg of protein in the crude extracts were analyzed as indicated in Materials and Methods. Bound fractions or crude cell extracts were analyzed by Western blot using the indicated antibodies. (B) HEK293 cells were transfected with the indicated plasmids and the ubiquitination of α-arrestin1 was analyzed as described in Materials and Methods. The ubiquitinated fractions and crude cell extracts (30 μg) were analyzed by Western blot using the indicated antibodies. In A and B, a representative blot of four independent experiments is shown. Molecular weight markers are indicated in kDa on the left of the blots. The bottom panel in (B) indicates the quantification of the ubiquitinated bands as described in Materials and Methods. Statistical significance between the samples is indicated as *p<0.05 and #p<0.05 (n:4).