Table 2. Molecular characterization of the Plant Defensins (PDF) genes in Arabidopsis thaliana and Brassica napus.
| Gene ID | Gene name | Block | CDS (bp) | Exon/ intron |
Amino acid (aa) |
Ka | Ks | Ka/Ks | Divergent time (Mya) |
|---|---|---|---|---|---|---|---|---|---|
| BnaA02g17500D | BnaA2.PDF1.1 | E | 243 | 2/1 | 80 | 0.1772 | 0.4305 | 0.4116 | 14.35 |
| BnaC02g23400D | BnaC2.PDF1.1a | E | 243 | 2/1 | 80 | 0.0586 | 0.3035 | 0.1930 | 10.11 |
| BnaC02g23420D | BnaC2.PDF1.1b | E | 243 | 2/1 | 80 | 0.1775 | 0.4289 | 0.4137 | 14.29 |
| BnaC06g22110D | BnaC6.PDF1.1 | E | 243 | 2/1 | 80 | 0.0993 | 0.3175 | 0.3128 | 10.58 |
| BnaA02g17510D | BnaA2.PDF1.2a | V | 249 | 2/1 | 82 | 0.2324 | 0.5643 | 0.4118 | 18.81 |
| BnaA06g30850D | BnaA6.PDF1.2a | V | 243 | 2/1 | 80 | 0.2157 | 0.4763 | 0.4529 | 15.87 |
| BnaC02g23410D | BnaC2.PDF1.2a1 | V | 249 | 2/1 | 82 | 0.2313 | 0.4939 | 0.4684 | 16.46 |
| BnaC02g23620D | BnaC2.PDF1.2a2 | V | 258 | 2/1 | 85 | 0.4893 | 0.7062 | 0.6929 | 23.54 |
| BnaC06g36530D | BnaC6.PDF1.2a | V | 243 | 2/1 | 80 | 0.0947 | 0.4695 | 0.2018 | 15.65 |
| BnaA02g17520D | BnaA2.PDF1.2b | I | 243 | 2/1 | 80 | 0.0749 | 0.3831 | 0.1956 | 12.77 |
| BnaA05g04680D | BnaA5.PDF1.2b1 | I | 243 | 2/1 | 80 | 0.5090 | 0.5004 | 1.0172 | 16.68 |
| BnaA05g04690D | BnaA5.PDF1.2b2 | I | 246 | 2/1 | 81 | 0.4842 | 0.4739 | 1.0218 | 15.79 |
| BnaA05g04760D | BnaA5.PDF1.2b3 | I | 243 | 2/1 | 80 | 0.4710 | 0.5987 | 0.7866 | 19.95 |
| BnaA07g21570D | BnaA7.PDF1.2b1 | I | 243 | 2/1 | 80 | 0.1011 | 0.3673 | 0.2753 | 12.24 |
| BnaA07g32130D | BnaA7.PDF1.2b2 | I | 243 | 2/1 | 80 | 0.0853 | 0.3282 | 0.2600 | 10.94 |
| BnaA07g32150D | BnaA7.PDF1.2b3 | I | 240 | 2/1 | 79 | 0.0705 | 0.3482 | 0.2023 | 11.60 |
| BnaC04g53180D | BnaC4.PDF1.2b1 | I | 243 | 2/1 | 80 | 0.5064 | 0.5078 | 0.9972 | 16.92 |
| BnaC04g53190D | BnaC4.PDF1.2b2 | I | 243 | 2/1 | 80 | 0.4662 | 0.6050 | 0.7706 | 20.16 |
| BnaC06g22140D | BnaC6.PDF1.2c | V | 240 | 2/1 | 79 | 0.0952 | 0.2719 | 0.3502 | 9.06 |
| BnaC06g22120D | BnaC6.PDF1.3 | I | 240 | 2/1 | 79 | 0.0868 | 0.3933 | 0.2206 | 13.11 |
| BnaA05g19430D | BnaA5.PDF1.4 | B | 288 | 3/2 | 95 | 0.1206 | 0.5015 | 0.2405 | 16.72 |
| BnaA08g22000D | BnaA8.PDF1.4 | B | 237 | 2/1 | 78 | 0.4702 | 3.1360 | 0.1499 | 104.53 |
| BnaC01g00250D | BnaC1.PDF1.4 | B | 231 | 2/1 | 76 | 0.4451 | 3.0059 | 0.1481 | 100.19 |
| BnaC06g33060D | BnaC6.PDF1.4 | B | 267 | 2/1 | 88 | 0.3904 | 5.6153 | 0.0695 | 187.17 |
| BnaC08g18880D | BnaC8.PDF1.4 | B | 237 | 2/1 | 78 | 0.0465 | 0.2858 | 0.1626 | 9.52 |
| BnaA02g26210D | BnaA2.PDF2.2 | K | 234 | 2/1 | 77 | 0.0572 | 0.5222 | 0.1096 | 17.40 |
| BnaA06g34320D | BnaA6.PDF2.2 | K | 234 | 2/1 | 77 | 0.0505 | 0.2948 | 0.1713 | 9.82 |
| BnaC02g47680D | BnaC2.PDF2.2 | K | 234 | 2/1 | 77 | 0.0572 | 0.5903 | 0.0969 | 19.67 |
| BnaC07g21510D | BnaC7.PDF2.2 | K | 231 | 2/1 | 76 | 0.0525 | 0.3780 | 0.1390 | 12.60 |
| BnaA09g18990D | BnaA9.PDF2.3 | K | 234 | 2/1 | 77 | 0.0743 | 0.3135 | 0.2371 | 10.45 |
| BnaC09g20670D | BnaC9.PDF2.3 | K | 234 | 2/1 | 77 | 0.0826 | 0.3291 | 0.2511 | 10.97 |
| BnaA02g33850D | BnaA2.PDF2.5 | X | 222 | 2/1 | 73 | 0.1430 | 0.8075 | 0.1771 | 26.91 |
| BnaC02g42620D | BnaC2.PDF2.5 | X | 222 | 2/1 | 73 | 0.1452 | 0.7109 | 0.2043 | 23.69 |
| BnaA06g34310D | BnaA6.PDF2.6 | K | 222 | 2/1 | 73 | 0.1761 | 0.3469 | 0.5076 | 11.56 |
| BnaC04g53620D | BnaC4.PDF2.6a | K | 222 | 2/1 | 73 | 0.4993 | 1.5605 | 0.3200 | 52.01 |
| BnaC04g07810D | BnaC4.PDF2.6b | K | 222 | 2/1 | 73 | 0.4988 | 1.7036 | 0.2928 | 56.78 |
| BnaC07g21520D | BnaC7.PDF2.6 | K | 222 | 2/1 | 73 | 0.1744 | 0.3814 | 0.4573 | 12.71 |
| AT1G75830 | AtPDF1.1 | E | 243 | 2/1 | 80 | ||||
| AT1G75830 | AtPDF1.2a | V | 243 | 2/1 | 80 | ||||
| AT2G26020 | AtPDF1.2b | I | 243 | 2/1 | 80 | ||||
| AT5G44430 | AtPDF1.2c | V | 243 | 2/1 | 80 | ||||
| AT2G26010 | AtPDF1.3 | I | 243 | 2/1 | 80 | ||||
| AT1G19610 | AtPDF1.4 | B | 237 | 2/1 | 78 | ||||
| AT1G55010 | AtPDF1.5 | C | 243 | 2/1 | 80 | ||||
| AT2G02120 | AtPDF2.1 | K | 234 | 2/1 | 77 | ||||
| AT2G02100 | AtPDF2.2 | K | 234 | 2/1 | 77 | ||||
| AT2G02130 | AtPDF2.3 | K | 234 | 2/1 | 77 | ||||
| AT1G61070 | AtPDF2.4 | D | 231 | 2/1 | 76 | ||||
| AT5G63660 | AtPDF2.5 | X | 222 | 2/1 | 73 | ||||
| AT2G02140 | AtPDF2.6 | K | 222 | 2/1 | 73 |
Note:
CDS, coding sequence; Ka, non-synonymous nucleotide substitution rate; Ks, synonymous nucleotide substitution rate.