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. 2021 Aug 12;16(9):871–891. doi: 10.2217/rme-2020-0202

Table 1. . Corneal endothelial cell markers.

CEC markers Location Function Method Remark Ref.
Na+/K+-ATPase Basolateral membrane Active transport pump WB, ICC, IHC, RT-PCR Found in epithelial cells, TM [27,30,31,33,34]
ZO-1 Apical submembraneous protein of the tight junction complex Intercellular tight junction WB, ICC, IHC, RT-PCR Found in epithelial cells [27,30,31,33]
VDAC3 Mitochondrial membrane channels Mitochondrial permeability transition pore RT-PCR None [33]
SLC4A4 Plasma membrane Na-HCO3 co-transporter, membrane transport protein RT-PCR None [33,34]
CLCN3 Plasma membrane Membrane transport protein RT-PCR None [33]
COL4A2 Extracellular matrix Produced by CECs RT-PCR None [34]
COL8A1, COL8A2 Extracellular matrix Produced by CECs RT-PCR, QPCR, WB DM component [34–36]
CDH2 Transmembrane Adherents junctions IHC, RT-PCR Specific for CECs [27,30,34]
CD98 Plasma membrane Membrane transport protein FCM None [30]
CD166 Basolateral transmembrane protein NA ICC, RT-PCR, FCM, IHC, IP Found in epithelial cells, stromal cells, TM [27,30–32,27]
CD340 Plasma membrane NA FCM None [30]
Integrin α3β1 Basal membrane NA IHC Found in epithelial cells [27]
CD56 (NCAM) Subapical lateral membrane Cell–cell adhesion during embryonic development IHC, FCM Found in stromal cells, TM [27,37]
Prdx-6 Lateral plasma membrane Role of antioxidant ICC, FCM, IP, IHC Found in TM [27,31,32,38]
CD248 Transmembrane NA FCM None [37]
SLC4A11 Transmembrane Na+/OH co-transport, Na+-independent H+ (OH-) transport, NH3 transport QPCR None [35,39]
CYYR1 Transmembrane NA QPCR None [35]

CEC: Corneal endothelial cell; DM: Descemet's membrane; FCM: Flow cytometry; ICC: Immunocytochemistry; IHC: Immunohistochemistry; IP: Immunoprecipitation; NA: Not available for corneal endothelial cells; QPCR: Quantitative PCR; RT-PCR: Reverse transcription PCR; TM: Trabecular meshwork; WB: Western blot.