Table 1. . Corneal endothelial cell markers.
CEC markers | Location | Function | Method | Remark | Ref. |
---|---|---|---|---|---|
Na+/K+-ATPase | Basolateral membrane | Active transport pump | WB, ICC, IHC, RT-PCR | Found in epithelial cells, TM | [27,30,31,33,34] |
ZO-1 | Apical submembraneous protein of the tight junction complex | Intercellular tight junction | WB, ICC, IHC, RT-PCR | Found in epithelial cells | [27,30,31,33] |
VDAC3 | Mitochondrial membrane channels | Mitochondrial permeability transition pore | RT-PCR | None | [33] |
SLC4A4 | Plasma membrane | Na-HCO3 co-transporter, membrane transport protein | RT-PCR | None | [33,34] |
CLCN3 | Plasma membrane | Membrane transport protein | RT-PCR | None | [33] |
COL4A2 | Extracellular matrix | Produced by CECs | RT-PCR | None | [34] |
COL8A1, COL8A2 | Extracellular matrix | Produced by CECs | RT-PCR, QPCR, WB | DM component | [34–36] |
CDH2 | Transmembrane | Adherents junctions | IHC, RT-PCR | Specific for CECs | [27,30,34] |
CD98 | Plasma membrane | Membrane transport protein | FCM | None | [30] |
CD166 | Basolateral transmembrane protein | NA | ICC, RT-PCR, FCM, IHC, IP | Found in epithelial cells, stromal cells, TM | [27,30–32,27] |
CD340 | Plasma membrane | NA | FCM | None | [30] |
Integrin α3β1 | Basal membrane | NA | IHC | Found in epithelial cells | [27] |
CD56 (NCAM) | Subapical lateral membrane | Cell–cell adhesion during embryonic development | IHC, FCM | Found in stromal cells, TM | [27,37] |
Prdx-6 | Lateral plasma membrane | Role of antioxidant | ICC, FCM, IP, IHC | Found in TM | [27,31,32,38] |
CD248 | Transmembrane | NA | FCM | None | [37] |
SLC4A11 | Transmembrane | Na+/OH− co-transport, Na+-independent H+ (OH-) transport, NH3 transport | QPCR | None | [35,39] |
CYYR1 | Transmembrane | NA | QPCR | None | [35] |
CEC: Corneal endothelial cell; DM: Descemet's membrane; FCM: Flow cytometry; ICC: Immunocytochemistry; IHC: Immunohistochemistry; IP: Immunoprecipitation; NA: Not available for corneal endothelial cells; QPCR: Quantitative PCR; RT-PCR: Reverse transcription PCR; TM: Trabecular meshwork; WB: Western blot.